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The most common cause of Vitamin B12 deficiency is a lack of intrinsic factor, a protein the stomach produces that is essential for B12 absorption

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Purification of GST-tagged proteins For protein expression, 400 mL bacterial cultures (DH5 or BL21 Codon Plus RIPL) were transformed with the corresponding expression plasmids, grown in LB medium supplemented with appropriate antibiotics and induced with 0.1 mM IPTG at OD 600 = 0.81.0