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Seasonal and interannual variability of the marine bacterioplankton community throughout the water column over ten years

2022;11:e13

L., Rovers, M

After alkylation, samples were washed twice with 3 times volumes of 6 M Urea (in 50 mM HEPES, pH 7.7) and once with 50 mM HEPES (pH 7.7) by centrifugation (5000 g for 30 min at 4 C) with 10 kDa molecular weight cut-off (MWCO) spin filters pre-rinsed, once with water (Sartorius AG, Gttingen, Germany)

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