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Hatzimouratidis K, Eardley I, Giuliano F, et al

Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

Symptoms are primarily digestive: intermittent bloating, sugar cravings, occasional loose stools, and mild fatigue