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Furthermore, when cells were pre-cultured in in DMEM supplemented with FBS that had been depleted of biotin using streptavidin, FLAG-tagged RELA became detectable in the absence of added biotin but was markedly diminished upon biotin supplementation (Supplementary Figure S4A)

For comparison of cell-type proportion and MFI (either mean, median, or geometric median fluorescent intensity based upon the distribution) values, a two-tailed Students t test was performed to indicate statistical significance

517 Energy metabolism in T2DM patients In T2DM, energy metabolism is disrupted, which is characterized by chronic hyperglycemia and impaired carbohydrate, fat, and protein metabolism