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This method assumes that similar molecules have similar MS fragmentation patterns, meaning that they tend to group closely together in a network, and is able to detect the spectral sets of related molecules (Hollywood et al., 2018)

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In some experiments, HEK-Blue Null1 as the parental cell line of HEK-Blue hTLR9 cells was used as a control, where cells pretreated with ENDO3 or ENDO12 (1 or 10 M) were stimulated with TNF (100 ng ml 1 )