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doi: 10.1098/rstb.2013.0227

Ice packs and an analgesic may be used, if required, to ease the discomfort

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Disclosures: Yayun Ren: HistoIndex Pte Ltd: Employee, Dean Tai: Histoindex.com: Employee, Elaine Chng: Nothing to Disclose, Kutbuddin Akbary: HistoIndex Pte Ltd: Employee, Nikolai Naoumov: Nothing to Disclose, Jonathan Fallowfield: Stimuliver: Advisor, Ipsen: Advisor, Kynos Therapeutics: Advisor, Resolution Therapeutics: Consultant, River 2 Renal Corp.: Advisor, Global Clinical Trial Partners: Advisor, Guidepoint: Consultant, GlaxoSmithKline: Grant/Research Support, Genentech: Grant/Research Support, Intercept Pharmaceuticals: Grant/Research Support, Timothy Kendall: Nothing to Disclose, David Kleiner: Nothing to Disclose, Arun Sanyal: Genfit, Akarna, Tiziana, Durect, Inversago, Hemoshear, North: Stock - publicly traded company, Astra Zeneca ( 60K), Boston Pharmaceutical ( 2) in efforts to improve clinical outcomes and prevent cirrhosis by performing early intervention

All other authors have no conflicts of interest to declare