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In vitro treatments To inhibit lysosomal or proteasomal activity, cells were treated overnight, starting on day 1 of chondrogenic differentiation, with 5 M chloroquine or 10 M MG132, respectively (both from Sigma-Aldrich) with dimethylsulfoxide (DMSO

Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine-N-Oxide

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Although to our knowledge, there is no direct evidence of crosstalk between the GDH pathway and the GABA shunt, we speculate that the GDH pathway may interfere with the decarboxylating step of the GABA shunt by controlling the available glutamate via the action of Gdh1, Gdh2 and Gdh3 proteins under specific conditions