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MT, as an endogenous antioxidant, is capable of crossing the BBB, scavenging free radicals, and upregulating the expression of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx), thereby enhancing antioxidant capacity (Banach et al., 2011)

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This issue can result in background signals and reduced specificity, making it difficult to accurately assess the target protein

The ATP standard curve was generated by replacing the 100-l bacteriaNF mixture with 100 l of tenfold serial dilutions of ATP in M9 medium (from 1 M to 10 pM), following the same assay procedure as used for the bacteriaNF samples