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Moreover, the absence of cortactin promotes epithelial apoptosis by increasing Ras homolog gene family, member A (RhoA)/ROCK1-dependent actin-myosin contraction, highlighting the importance of cytoskeletal dynamics in barrier maintenance [72]

Areas Mol

Methods 25, 402408 (2001)

References: Leikin, JB et al (2002): A review of nerve agent exposure for the critical care physician

Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s