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Russell RM, Baik H, Kehayias JJ

[58] found that Raman spectral profiles of extracellular vesicles could differentiate severe ME/CFS donors and healthy controls (AUC = 0.7), but to achieve a rate of 100% true positives, classifiers would also allow a high rate of false positives

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This is because the pH of many of these stringent washes are quite extreme, so you want to reestablish a more neutral pH before long term storage of the beads