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Alternatively, we also optimized the cell sorting procedure by separating the bead/cell complexes through free settling instead of using the magnetic stand, as described previously ( RNA extraction and RT-qPCR Total RNA was extracted using the GeneJET RNA Purification Kit (K0732, Thermo) according to the manufacturers instructions

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A 100x dilution of the reaction was made and the activity was determined immediately and after 60 min

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In the extracellular environment, the identification of these proteins by cells of the immune system results in signal transduction that leads to the release of proinflammatory cytokines, such as IFN