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GPX4 knockdown GPX4 was suppressed in metM-Wnt lung cells by lentiviral transduction for 48 h, using particles generated by transfection of HEK293T cells with psPAX2, pMD2.G, and GPX4-targeting shRNAs in a psi-LVRU6H vector (Genecopoeia, GCACGAATTCTCAGCCAAGGA [shGPX4-A], GCTGGGAAATGCCATCAAATG [shGPX4-C], and GCTTCGCGCCGTAGTCTTA [Scram]) and were selected for using hygromycin (500 g/ml)

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Further studies have however shown that genetic aberrations present in hiPSC clones are not de novo mutations generated during the reprogramming process, but are most likely derived from the original somatic cells from which the hiPSC were reprogrammed 41