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Data hinted that surgical manipulations of the intestines might markedly dampen the contractile responses to both inducements

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The RNA and primers were then annealed by incubating as follows: 90 C (1 min), 80 C (1 min), 70 C (1 min), 60 C (1 min), 50 C (1 min), and 40 C (6 min)

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