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After intervention, the original medium was discarded, and 1 mL of 10 mol/L DCFH-DA working solution prepared in serum-free DMEM was added to each well to ensure complete coverage of the cells

To avoid interference, double blank (blank matrix only) and QC (blank matrix spiked with internal standard only) were also prepared and analyzed

Androgens alleviate allergic airway inflammation by suppressing cytokine production in Th2 cells

The rats in the fed group had unlimited access to food, whereas those in the fasted group were deprived of food for 18 h prior to the start of the experiment