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Culture conditions Slices were maintained in incubators at 37 C, under a humidified atmosphere containing 5% CO2 in six-well plates containing 1 ml of slice culture medium, containing basal Earles salts medium (BME, sigma, MFCD00217343), 25% Hanks balanced salt solution (Sigma, H9394), 27 mM glucose (Sigma, G8769), 1% penicillin/streptomycin (Life technology, 15140122), 1 mM glutamine (Sigma, 228034), and 5% horse serum (New Zealand origin, heat-inactivated