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Given that both cysteine and GSH contribute to cell metabolism, which may be a confounding factor in MTT assay, we verified the extent of cell death by propidium iodide (PI) staining

224 Lipid-derived PPAR ligands PPARs are involved in modulating multiple biological processes, including glucose and lipid metabolism, the inflammatory response, and cell proliferation

doi:10.1016/j.cgh.2017.04.016

It supports the biosynthesis of acetylcholine, which is the most abundant neurotransmitter in the body, regulating activities of vital organs and blood vessels, and the communication between nerves and muscles

To deactivate any enzymatic activity, the supernatant was heated to 100 C for 10 min, then allowed to return to ambient temperature, and mixed in a 1:1 ratio with 50 mL of isopropanol