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To exclude NADP + level, the supernatant was incubated at 60 C for 30 min and then 50 l supernatant was mixed with 98 l NADP cycling buffer and 2 l NADP cycling enzyme for 5 min incubation at room temperature

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Thermal cycler conditions are as follows: 98 C for 5 min, 30 cycles of 98 C for 15 s, 60 C for 60 s, and 72 C for 180 s