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There is a dynamic interplay of pulsatile GHRH and somatostatin secretion: Via crosstalk : GHRH neurons receive inhibitory inputs from somatostatin neurons, whilst somatostatin neurons receive direct stimulatory inputs from GHRH neurons Via synergistic actions on somatotrophs : Pre-exposure to somatostatin enhances GHRH-stimulated secretion of GH (457)

Crucially, the researchers explored substituting these expensive growth factors with a small-molecule cocktail, specifically 10 g/mL Vitamin C, 0.1 M Dihexa, and 10 M Forskolin (VDF cocktail), to assess its efficacy in inducing hepatic specification