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Worth noting here is that the induction of sufficient damage to non-nuclear cellular components to evoke upfront necrosis may require clinically-irrelevant high doses (Warters et al., 1978)

This improvement of yield may be largely attributed to enrichment of the extracts with nutrients, stimulating biochemical processes in plants, and consequently by boosting the translocation and concentration of certain metabolites in plant organs leading to accumulating dry matter, a greater number of pods per plant, seeds per pod, and pod weight are produced, which are the major parameters in assessing the yield of bean

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Proteins were boiled with laemmli sample buffer (Biorad) plus 2-mercaptoethanol as a loading buffer