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After reconstruction and voxel placement (3 3 3 mm 3 ), an MRS water reference was acquired for each animal, followed by STEAM sequence (TR 1500 ms, TE = 3 ms, TI = 10 ms, spatial-water-fat shift = 0.467 mm, spectroscopy number of points = 2048, spectral width = 4006.41 Hz, water suppression scheme = VAPOR, bandwidth 200 Hz, outer volume suppression = ON, OVS slice thickness = 10 mm, gap to voxel = 0 mm, number of averages = 512, scanning time = 12.8 min) in the right hemisphere

PMID: 30632091.