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Description

Homeostasis of thymus-derived foxp3+ Regulatory T cells is controlled by ultraviolet B exposure in the skin

The cells were then digested with trypsin at 37C, centrifuged at 300 g for 5 min, washed with cold-PBS three times and incubated in the dark at 37C with 20 l annexin V-fluorescein isothiocyanate antibody and PI staining solution for 15 min