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The experiment was set up with six treatments, six replicates per treatment, and 60 fish per replicate

Pro-inflammatory cytokines such as TNF- , IL-1, and IL-6 can enhance BBB permeability by altering tight junction proteins, resulting to a loss of barrier integrity

doi: 10.1016/j.cryobiol.2012.06.007 117

Oral administration of supplements to restore brain glutathione and l-cysteine levels proves utterly fruitless given that GSH and l-cysteine undergo rapid degradation and display only partial transit across the cerebral vasculature barrier

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