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Equal amounts of protein were separated by 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes

DMF was also shown to diminish the metabolic flux of glutamine to the tricarboxylic acid (TCA) cycle and divert it towards GSH biosynthesis 26

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Although the body produces some of its own glutathione, poor diet, smoking, air pollution, stress, infections and radiation reduce the proportion of glutathione in the body