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If a protocol contemplates delivering, say, 250 mcg intranasally, the 5 mg/mL dilution places that in 0.05 mL a realistic single actuation whereas the 1 mg/mL dilution would require 0.25 mL, likely split across multiple drops or nostrils

Menp et al

Vacuolar localization of secondary metabolites has been suggested long time ago but it was experimentally demonstrated using water-free environment gradient centrifugation of Arabidopsis leaf tissue only recently ( Figure 2 , transporters 10 and 11) and we consider them below

Intranasal delivery of cells to the brain