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Preclinical studies employ highly variable citrulline dosages, including dietary incorporation (0.6%-1%) ( in vitro concentrations (12 mM) ( 5.3 Efficacy evaluation Regarding outcomes, while current research effectively documents improvements in intermediate endpoints like sperm motility, mitochondrial membrane potential, and DNA fragmentation ( Current research on L-citrullines protective mechanisms remains largely confined to conventional biomarkers, such as OS markers (e.g., ROS, SOD) and mitochondrial function parameters ( 5.4 Gut-testis axis In recent years, the gut microbiota-testicle axis has recently emerged as a critical frontier in reproductive biology