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Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate

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The asarone in VOA is found to be principally responsible for the toxic effects of VOA

Therefore, once again, a combination of the two ligands may be thought to bind with more affinity to the enzyme