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Lipid peroxidation is known to be one of the mo- lecular mechanisms for cell injury in acute mercury poi- soning and is associated with a decrease in cellular anti- oxidants such as glutathione ascorbate, superoxide dis- mutase (SOD) and catalase (CAT) [55,56]

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Post-treatment, cells were collected and washed 3x with ice-cold PBS, and lysed in 250 l of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1% Triton-X-100, 1 mM EDTA, 5 mM MgCl2, 5% glycerol) freshly supplemented with the 100X Halt Protease Inhibitor Cocktail for 20 min on ice

doi: 10.1038/nrm1890 223 ViningKHMooneyDJ

however, because of the special identification method, not all samples were screened for hCG (Table 2)