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The sections were then washed with PBS and treated with secondary antibody (diluted in PBS containing 0.3% Triton X-100) for 2 h at room temperature

Buprenorphine can produce miosis and changes in the level of consciousness that may interfere with patient evaluation

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Don't share space with food, don't open it more than once daily, and don't assume your kitchen refrigerator maintains consistent temperatures just because the milk hasn't spoiled