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Thymosin Alpha-1 has also been shown to enhance vaccine response and help people with severe COVID possibly by repairing damage caused by inflammation

Jhunjhunwala S, Hammer C, Delamarre L

Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)