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Thus, the conjugate is actively carried by a transporter protein across the bacterial membrane, where it is cleaved by a dipeptidase enzyme, and then a -lyase releases the sulfur compound

Intein-mediated protein processing and labeling EPL and PTS have been successfully used to produce diverse N- and C-terminal modifications of target proteins that are expertly reviewed in the recent literature[7, 8, 10, 12], including protein phosphorylation, lipidation, glycosylation, biotinylation, ubiquitination, and segmental isotope labeling