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Pb significantly changes the blood parameters of fish [172,173,174,175,176]

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Invasive methods that primarily rely on disruption of the BBB integrity by osmotic or biochemical means, or direct intracranial drug delivery by intracerebroventricular, intracerebral or intrathecal administration after creating reversible openings in the head are recognized [54]

This effect was achieved by increasing the phosphorylation of GSK3 and decreasing the expression of DKK-1

Cryo-EM imaging was performed on a Titan Krios at 300 kV accelerating voltage in the Center of Cryo-Electron Microscopy Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (Shanghai, China)