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After removing unbound antibodies, blank wells were treated with BSA enzyme inhibitor, and macrophage supernatant was added

Morales DK, Grahl N, Okegbe C, Dietrich LE, Jacobs NJ, Hogan DA

Neurology 65(4):586590

Co-exposure of cardiomyocytes to IFN- and TNF- induces mitochondrial dysfunction and nitro-oxidative stress: implications for the pathogenesis of chronic chagas disease cardiomyopathy