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Following Matrigel polymerization for 10 min at 37 C, advanced DMEM/F12 was supplemented with penicillin/streptomycin, 2 mM GlutaMAX, 10 mM HEPES, 1 B27, 1 N2 (Life Technologies), 1mM N-acetylcysteine (Sigma), and 10 nM gastrin I (Biogems)

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In rare cases, a long-standing B12 deficiency can affect the optic nerve, potentially causing blurred or double vision

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Anxiety does not always cause nerve pain directly, but it can heighten sensitivity in the nervous system, making existing discomfort worse and sometimes even triggering new sensations