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HPLC high performance liquid chromatography Neuroblastoma SHSY5Y (NB) cells were maintained in Dulbeco's Modified Eagle's Medium (DMEM), supplemented with 8% FCS and 8% horse serum, penicillin (25 g/ml), streptomycin (25 g/ml), 2 mM L-glutamine and 400 g/ml G418 (Gibco/BRL)

The protein content of each sample was determined using the Bradford (1976) method and a standard of bovine serum albumin

For example, NR is phosphorylated by nicotinamide riboside kinase (NRK) to form NMN, while NAM is catalyzed by nicotinamide phosphoribosyltransferase (NAMPT) to transform into NMN (Imai, 2011)

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ROS damages mitochondrial membrane integrity and mt-DNA, resulting in mitochondrial dysfunction