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The germination and initial growth stages of seedlings are among the most vulnerable phases of plant life cycle to heavy metals ( 2 fixation and ATP production ( In contrast, plants treated with ALA showed significant improvement in growth parameters under Pb stress

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Briefly, free Lf was removed using centrifugation with an ultracentrifugal filter (100 kDa MWCO, Amicon ) for 1 h at 4 500 g

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