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T.PopaS.et al (2023)

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doi: 10.1242/dev.01128 4 AlbillosS

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The H 2 O 2 remaining after incubation with CAT was determined by mixing 5 L of the reaction mix with 100 L of H 2 O 2 determination reagent (contain 1 g/L 2,2-azino-bis(3-ethylbenothiazoline-6-sulphonic acid, 0.8 U/mL horseradish peroxidase from Sigma) in the well of a new microplate, incubating the plate for 10 min at 20 o C, and measuring the absorbance at 410 nm