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The partial coating of SP with macrophage membranes in our study was consistent with the findings reported in previous literature 33

AMS binds to reduced thiol groups in Cys residues and increases the molecular mass of the protein, so that the reduced and oxidized forms of GSAAT are readily distinguishable on SDS-PA gels, since the labeled protein (reduced form) migrates more slowly than the unlabeled oxidized form

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That logic doesnt apply here