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Abnormal redox balance, as measured by higher BAL fluid oxidized glutathione (GSSG) and lower intraepithelial cell GSH, is observed in asthmatic BAL fluid, as compared with healthy controls

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The reaction was stopped by the addition of 3 N H 3 PO 4 , followed by measurement of the absorbance using a VersaMax ELISA reader (Molecular Devices, Temecula, CA, USA) at the wavelength of 450 nm, corrected to the absorbance at the wavelength of 540 nm