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For generating calibration curve of HT1080 cells, exact same conditions were used as described above

Mitochondrial respiration rates (pmols 1 ) were normalized for mitochondrial suspension protein levels (pmols 1 g 1 ) in the isolated mitochondria preparation using the Pierce 660 nm protein assay (Thermo Scientific) to obtain the intrinsic mitochondrial respiration, which is an index of mitochondrial quality

The main targets in these models are thyroid follicular cells, immune cells (particularly T lymphocytes), and the autoantibodies that bind to thyroid antigens

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