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Covalent and non-covalent binding of fluorescein-isothiocyanate and fluorescein to proteins

In AP, damage-associated molecular patterns (DAMPs) released from necrotic acinar cells, such as mitochondrial DNA, extracellular ATP, and reactive oxygen species (ROS), which activated NLRP3 inflammasome and resulted in perpetuating a vicious cycle of inflammation, pancreatic necrosis, and systemic inflammatory response syndrome (Papantoniou et al., 2024)

The identification and purification of active ingredients after the fermentation of alternative raw materials, the metabolic pathways in kombucha, and the specific pharmacological mechanisms of action have not yet been extensively studied

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