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The mTOR signaling can be activated by three board categories of instructive signals, encompassing environmental cues (e.g., growth factors and immunoregulatory factors), immune signals (e.g., co-stimulation, antigens, and cytokines), and nutrients (e.g., essential amino acids and glucose) (98)

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Because cell viability and apoptosis were not directly assessed in this study, it is difficult to conclude if caspase-3 activation was accompanied by increased apoptosis (Bonnard et al., 2008)