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After several rinses with 0.01 M PBS, the sections were placed in secondary antibody (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 2 h

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Finally, the MS/MS experiments were carried out in flow injection analysis mode for AAs and ACs and in LC for MMA and lactic acid (LA) using an API 4000 triple quadrupole mass spectrometer (Sciex) coupled with a 1160 series Agilent HPLC system (Agilent Technologies) (45, 46)

When NAD+ levels are adequate, SIRT1 activity increases, promoting DNA repair, reducing inflammation, and enhancing stress resistance