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6 Its a vicious cycle, unfortunately

The reaction was initiated by mixing 2035 g protein with a reaction mixture containing 10 mM ATP, 5 mM L-cysteine, 50 mM L-glutamate, 500 M acivicin, 20 mM MgCl 2 , 100 mM Tris-HCl pH 8.2 in a total volume of 150 l followed by incubation for 15 min at 25C

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doi: 10.1159/000360101 154