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Increasing amounts of NADPH (1250 M) were subsequently added to the wells and the solutions were imaged on an LSM880 confocal laser scanning microscope (Carl Zeiss AG, Oberkochen, Germany) with a 63x immersion objective (LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27) using a 440 nm pulsed excitation laser (LDH-D-C-440, 40 MHz repetition rate) managed by a PicoQuant FLIM module (Sepia PDL828-S, PMA Hybrid 40, MultiHarp 150 4N, Time Harp 260 PICO Dual) (PicoQuant, Berlin, Germany)

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Tumor cells in the TME require more exogenous non-essential amino acids for cellular activities 133

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Paraquat-treated mice group has higher ROS generation as compared to the control group which can be seen via Dichlorofluorescein (DCF) fluorescence intensity