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This process appears to be independent of PKA or AR stimulation (Gao et al., 2008)

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Subsequently, they were incubated with 0.5% Triton X-100 for 10 min, blocked with PBS containing 3% BSA for 1 h, and incubated overnight at 4 C with the following primary antibodies: anti-Laminin (#PA1-16730, Thermofisher), anti-PAX7 (#PA1-117, Thermofisher), and anti-Perilipin (#PA1-1051, Thermofisher)

More importantly, there are several aspects of irAEs that we do not understand and which are the source of several controversies in the field