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Acknowledgements Work in the authors laboratories was supported by Ministerio de Ciencia e Innovacin (MCIN), Agencia Estatal de Investigacin (AEI) MCIN/AEI/10.13039/501100011033 grant PID2020-113521RB-I00

observed the repressed expression of GPX4 in the mitochondria of RGCs, concomitant with elevated levels of mt-ROS (96)

Consistency: Antioxidant levels build up over time

The collected sample aliquots were immediately frozen in liquid nitrogen and stored at -80C until further processing

As the first line of immune defense in the CNS, microglia mediate neuroinflammatory responses by releasing pro-inflammatory cytokines such as interleukin-1 (IL-1), tumor necrosis factor- (TNF-), and interleukin-6 (IL-6), disrupting neuronal homeostasis and accelerating the development of morphine tolerance ( Currently, no drugs have been approved for clinical use specifically to prevent or reverse morphine tolerance