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Description

Following treatment, media was replaced with 80% (v/v) methanol (cooled to 80 C.) containing 500 nmol 6-chloronicotinamide (6-CN) as an internal standard (IS) to extract cellular metabolites

What to Do if You Receive a False Positive Result

In practice, gelling usually traces back to three avoidable errors: Diluent that is too cold: Cold liquid can create an abrupt hydration shock

Indeed, B12 and folate interact in the metabolism of methyl group in the transform from 5-MTHF to homocysteine, and this reaction is mediated by B12 dependent methltransferase, which results in the formation of methionine