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Methods: Male C57/BL6J mice aged 8-9 week (wk) aged with subcutaneous implanted osmotic pump delivering 2.5mmol/kg/d of ammonium acetate (AmAc) or vehicle (PBS) for 6 wk were randomized to either voluntary wheel running (VWR) or usual activity (UA) for 4wk

As autofluorescence can rapidly increase in consequence to plant immune responses (Bohle et al ., 2024 a ), roGFP2 fluorescence-based read-out, especially after excitation in the UV range (e.g