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Equal amounts of protein from each sample were subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the separated proteins were transferred to PVDF membranes

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While this can be due to asthma that has persisted since childhood, most often asthma in the elderly involves neutrophil infiltration of the airways and can be driven by T1 or TH17 pathways (T2-low)