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Briefly, the cells were resuspended in hydrogel precursor solution containing 2 mM norbornene-functionalized 4-arm 20 kDa PEG-amine macromers, 4 mM of the dicysteine functionalized MMP degradable peptide (GKKCGPQG IWGQCKKG), 0.05 wt% of the photoinitiator lithium phenyl-2,4,6- trimethylbenzoylphosphinate (LAP) 41 , and 0.1 mg/mL laminin in PBS 4,5,19,42

These cultivated materials did not require specific collection permits, and no voucher specimens were deposited

2013 AHA/ACC/TOS guideline for the management of overweight and obesity in adults: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines and The Obesity Society

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